Saska et.al, 2016
Pavel Saska, Jiří Skuhrovec, Jan Lukáš, HsinChi, Shu-JenTuan & Alois Honěk, “Treatment by glyphosate-based herbicide alters life history parameters of the rose-grain aphid Metopolophium dirhodum,” Nature: Scientific Reports, 2016, 6:27801, DOI: 10.1038/srep27801.
ABSTRACT:
Glyphosate is the number one herbicide in the world. We investigated the sub-lethal effects of this herbicide on the aphid Metopolophium dirhodum (Walker), using an age-stage, two-sex life table approach. Three concentrations of the herbicide (low – 33.5, medium – 66.9 and high – 133.8mmol dm−3 of active ingredient) and distilled water as the control were used. The LC50 of the IPA salt of glyphosate on M. dirhodum was equivalent to 174.9mmol dm−3 of the active ingredient (CI95: 153.0, 199.0). The population parameters were significantly negatively affected by herbicide application, and this negative effect was progressive with the increasing concentration of the herbicide. A difference of two orders of magnitude existed in the predicted population development of M. dirhodum between the high concentration of the herbicide and the control. This is the first study that comprehensively documents such a negative effect on the population of an herbivorous insect. FULL TEXT
Hansen and Rosley, 2016
Lone Rykær Hansen, Peter Roslev, “Behavioral responses of juvenile Daphnia magna after exposure to glyphosate and glyphosate-copper complexes,” Aquatic Toxicology, 2016, 179: 36-43, DOI: 10.1016/j.aquatox.2016.08.010.
ABSTRACT:
Glyphosate (N-(phosphonomethyl)glycine) is the active ingredient in a range of popular broad-spectrum herbicide formulations. Glyphosate is a chelating agent that can form stable complexes with divalent metal ions including Cu(II). Little is known about the bioavailability and ecotoxicity of glyphosateCu(II) complexes to aquatic organisms. In this study, we used video tracking and behavior analysis to investigate sublethal effects of binary mixtures of glyphosate and Cu(II) to juvenile D. magna. Behavioral responses were quantified for individual D. magna after 24 h and 48 h exposure to glyphosate and glyhosate-Cu(II) mixtures. Sublethal concentrations resulted in decreases in swimming velocity, acceleration speed, and distance moved whereas inactive time of D. magna increased. Distance moved and inactive time were the most responsive parameters to glyphosate and glyphosate-Cu(II) exposure. On a molar basis, glyphosate-Cu(II) complexes appeared more toxic to D. magna than glyphosate alone. The 48 h EC50 for glyphosate and glyphosate-Cu(II) determined from swimming distance were 75.2 M and 8.4 M, respectively. In comparison, traditional visual observation of mobility resulted in 48 h EC50 values of 52.8 M and 25.5 M for glyphosate and glyphosate-Cu(II), respectively. The behavioral responses indicated that exposure of D. magna to mixtures of glyphosate and Cu(II) attenuated acute metal toxicity but increased apparent glyphosate toxicity due to complexation with Cu(II). The study suggests that glyphosate is a likely mediator of aquatic metal toxicity, and that video tracking provides an opportunity for quantitative studies of sublethal effects of pesticide complexes. FULL TEXT
Sol Balbuena et. al, 2015
María Sol Balbuena, Léa Tison, Marie-Luise Hahn, Uwe Greggers, Randolf Menzel, and Walter M. Farina, “Effects of sub-lethal doses of glyphosate on honeybee navigation,” Journal of Experimental Biology,
ABSTRACT:
Glyphosate (GLY) is a herbicide that is widely used in agriculture for weed control. Although reports about the impact of GLY in snails, crustaceans and amphibians exist, few studies have investigated its sublethal effects in non-target organisms such as the honeybee Apis mellifera, the main pollen vector in commercial crops. Here, we tested whether exposure to three sublethal concentrations of GLY (2.5, 5 and 10 mg l−1: corresponding to 0.125, 0.250 and 0.500 μg per animal) affects the homeward flight path of honeybees in an open field. We performed an experiment in which forager honeybees were trained to an artificial feeder, and then captured, fed with sugar solution containing traces of GLY and released from a novel site either once or twice. Their homeward trajectories were tracked using harmonic radar technology. We found that honeybees that had been fed with solution containing 10 mg l−1 GLY spent more time performing homeward flights than control bees or bees treated with lower concentrations. They also performed more indirect homing flights. Moreover, the proportion of direct homeward flights performed after a second release from the same site increased in control bees but not in treated bees. These results suggest that, in honeybees, exposure to levels of GLY commonly found in agricultural settings impairs the cognitive capacities needed to retrieve and integrate spatial information for a successful return to the hive. Therefore, honeybee navigation is affected by ingesting traces of the most widely used herbicide worldwide, with potential long-term negative consequences for colony foraging success. FULL TEXT
Gaupp-Berghausen et. al, 2015
Mailin Gaupp-Berghausen, Martin Hofer, Boris Rewald, and Johann G. Zaller, “Glyphosate-based herbicides reduce the activity and reproduction of earthworms and lead to increased soil nutrient concentrations,” Nature: Scientific Reports, 2015, 5: 12886, DOI: 10.1038/srep12886.
ABSTRACT:
Herbicide use is increasing worldwide both in agriculture and private gardens. However, our earthworms, is still very scarce. In a greenhouse experiment, we assessed the impact of the most strategies. We demonstrate, that the surface casting activity of vertically burrowing earthworms (Lumbricus terrestris) almost ceased three weeks after herbicide application, while the activity of soil dwelling earthworms (Aporrectodea caliginosa) was reduced by 56% within three months after herbicide application. Herbicide application led to increased soil concentrations of nitrate by 1592% and phosphate by 127%, pointing to potential risks for nutrient leaching into streams, lakes, or groundwater aquifers. These sizeable herbicide-induced impacts on agroecosystems are particularly worrisome because these herbicides have been globally used for decades. FULL TEXT
Ryberg and Gilliom, 2015
Karen R. Ryberg and Robert J. Gilliom, “Trends in pesticide concentrations and use for major rivers of the United States,” Science of the Total Environment, 2015, 538: 431-444, DOI: /10.1016/j.scitotenv.2015.06.095.
ABSTRACT:
Trends in pesticide concentrations in 38 major rivers of the United States were evaluated in relation to use trends for 11 commonly occurring pesticide compounds. Pesticides monitored in water were analyzed for trends in concentration in three overlapping periods, 1992–2001, 1997–2006, and 2001–2010 to facilitate comparisons among sites with variable sample distributions over time and among pesticides with changes in use during different periods and durations. Concentration trends were analyzed using the SEAWAVE-Q model, which incorporates intra-annual variability in concentration and measures of long-term, mid-term, and short-term
streamflow variability. Trends in agricultural use within each of the river basins were determined using interval-censored regression with high and low estimates of use.
Pesticides strongly dominated by agricultural use (cyanazine, alachlor, atrazine and its degradate deethylatrazine, metolachlor, and carbofuran) had widespread agreement between concentration trends and use trends. Pesticides with substantial use in both agricultural and nonagricultural applications (simazine, chlorpyrifos, malathion, diazinon, and carbaryl) had concentration trends that were mostly explained by a combination of agricultural-use trends, regulatory changes, and urban use changes inferred from concentration trends in urban streams. When there were differences, concentration trends usually were greater than use trends (increased more or decreased less). These differences may occur because of such factors as unaccounted pesticide uses, delayed transport to the river through groundwater, greater uncertainty in the use data, or unquantified land use and management practice changes. FULL TEXT
Brodeur et. al, 2016
Julie Celine Brodeur, Solene Malpel, Ana Belen Anglesio, Diego Cristos, María Florencia D’Andrea, María Belen Poliserpi, “Toxicities of glyphosate- and cypermethrin-based pesticides are antagonic in the tenspotted livebearer fish (Cnesterodon decemmaculatus),” Chemosphere, 2016, 155:429-435, DOI: 10.1016/j.chemosphere.2016.04.075.
ABSTRACT:
Although pesticide contamination of surface waters normally occurs in the form of mixtures, the toxicity and interactions displayed by such mixtures have been little characterized until now. The present study examined the interactions prevailing in equitoxic and non-equitoxic binary mixtures of formulations of glyphosate (Glifoglex®) and cypermethrin (Glextrin®) to the tenspotted livebearer (Cnesterodon decemmaculatus), a widely distributed South American fish. The following 96 h-LC50s were obtained when pesticide formulations were tested individually: Glifoglex® 41.4 and 53 mg ae glyphosate/L; Glextrin® 1.89 and 2.60 mg cypermethrin/L. Equitoxic and non-equitoxic mixtures were significantly antagonic in all combinations tested. The magnitude of the antagonism (factor by which toxicity differed from concentration addition) varied between 1.37 and 3.09 times in the different non-equitoxic mixtures tested. Antagonism was due to a strong inhibition of cypermethrin toxicity by the glyphosate formulation, the toxicity of the cypermethrin-based pesticide being almost completely overridden by the glyphosate formulation. Results obtained in the current study with fish are radically opposite to those previously observed in tadpoles where synergy was observed when Glifoglex® and Glextrin® were present in mixtures. FULL TEXT
Ma et. al, 2015
Junguo Ma, Yanzhen Bu, Xiaoyu Li, “Immunological and histopathological responses of the kidney of common carp (Cyprinus carpio L.) sublethally exposed to glyphosate,” Environmental Toxicology and Pharmacology, 2015, 39: 1-8, DOI: 10.1016/j.etap.2014.11.004.
ABSTRACT:
Glyphosate is a broad-spectrum herbicide frequently used world widely in agricultural and non-agricultural areas to control unwanted plants. Health risk of chronic and subchronic exposure of glyphosate on animals and humans has received increasing attention in recent years. The aim of this study was to evaluate the effects of glyphosate on the immunoglobulin M (IgM), complement C3 (C3), and lysozyme (LYZ) in the kidney of common carp exposed to 52.08 or 104.15mg L−1 of glyphosate for 168h. The results showed that the transcriptions of IgM, C3, or LYZ were altered due to glyphosate-exposure, for example, IgM and C3 initially increased at 24h later it decreased (except for a increase of C3 in higher dose group at 24h) while the expression of G-type LYZ were not affected at 24h, then increased at 72h, but decreased at the end of test, however C-type LYZ expression was initially up-regulated (24–72h) but down-regulated at the end of exposure (168h). However, glyphosate-exposure generally decreased the contents of IgM and C3 or inhibited LYZ activity in the kidney of common carp. In addition, glyphosate-exposure also caused remarkable histopathological damage, mainly including vacuolization of the renal parenchyma and intumescence of the renaltubule in fish kidney. The results ofthis study indicate that glyphosate causes immunotoxicity on common carp via suppressing the expressions of IgM, C3, and LYZ and also via damaging the fish kidney. FULL TEXT
Brodeur et. al, 2014
Julie Céline Brodeur, María Belén Poliserpi, María Florencia D’Andrea, Marisol Sánchez, “Synergy between glyphosate- and cypermethrin-based pesticides during acute exposures in tadpoles of the common South American Toad Rhinella arenarum,” Chemosphere, 2014, 112:70-76, DOI: 10.1016/j.chemosphere.2014.02.065.
ABSTRACT:
The herbicide glyphosate and the insecticide cypermethrin are key pesticides of modern management in soy and corn cultures. Although these pesticides are likely to co-occur in ephemeral ponds or aquatic systems supporting amphibian wildlife, the toxicological interactions prevailing in mixtures of these two pesticides have been little studied. The current study evaluated the toxicity of equitoxic and nonequitoxic binary mixtures of glyphosate- and cypermethrin-based pesticides to tadpoles of the common South American toad, Rhinella arenarum. Two different combinations of commercial products were tested: glyphosate Glifosato Atanor + cypermethrin Xiper and glyphosate Glifoglex + cypermethrin Glextrin. When tested individually, the formulations presented the following 96 h-LC50s: Glifosato Atanor 19.4 mg ae L1 and Glifoglex 72.8 mg ae L1 , Xiper 6.8 mg L1 and Glextrin 30.2 mg L1. Equitoxic and non-equitoxic mixtures were significantly synergic in both combinations of commercial products tested. The magnitude of the synergy (factor by which toxicity differed from concentration addition) was constant at around twofold for all tested proportions of the glyphosate Glifoglex + cypermethrin Glextrin mixture; whereas the magnitude of the synergy varied between 4 and 9 times in the glyphosate Glifosato Atanor + cypermethrin Xiper mixture. These results call for more research to be promptly undertaken in order to understand the mechanisms behind the synergy observed and to identify and quantify the extent of its environmental impacts. FULL TEXT
Relyea, 2011
Rick A. Relyea, “Amphibians Are Not Ready for Roundup®,” in Wildlife Ecotoxicology: Forensic Approaches, J.E. Elliott et al. (eds.), 2011, DOI 10.1007/978-0-387-89432-4_9.
ABSTRACT:
The herbicide glyphosate, sold under a variety of commercial names including Roundup® and Vision® , has long been viewed as an environmentally friendly herbicide. In the 1990s, however, after nearly 20 years of use, the first tests were conducted on the herbicide’s effects on amphibians in Australia. The researchers found that the herbicide was moderately toxic to Australian amphibians. The leading manufacturer of glyphosate-based herbicides, Monsanto, declared that the researchers were wrong. Nearly 10 years later, my research group began examining the effects of the herbicides on North American amphibians. Based on an extensive series of experiments, we demonstrated that glyphosate-based herbicides can be highly toxic to larval amphibians. Monsanto declared that we were also wrong. These experiments have formed the basis of a spirited debate between independent, academic researchers, and scientists that either work as consultants for Monsanto or have a vested interest in promoting the application of the herbicide to control undesirable plants in forests and agriculture. The debate also moved into unexpected arenas, including the use of glyphosate-based herbicides in the Colombian drug war in South America where a version of Roundup is being used to kill illegal coca plantations. In 2008, the US EPA completed a risk assessment for the effects of glyphosate-based herbicides on the endangered California red-legged frog (Rana aurora draytonii) and concluded that it could adversely affect the long-termpersistence of the species. More recent data from Colombia have confirmed that the herbicides not only pose a risk to tadpoles in shallow wetlands, but that typical applications rates also can kill up to 30% of adult frogs. As one reflects over the past decade, it becomes clear that our understanding of the possible effects of glyphosatebased herbicides on amphibians has moved from a position of knowing very little and assuming no harm to a position of more precise understanding of which concentrations and conditions pose a serious risk. FULL TEXT
Mahler et. al, 2017
Mahler BJ, Van Metre PC, Burley TE, Loftin KA, Meyer MT, Nowell LH, “Similarities and differences in occurrence and temporal fluctuations in glyphosate and atrazine in small Midwestern streams (USA) during the 2013 growing season,” Science of the Total Environment, 2017 ,579:149-158. DOI: 10.1016/j.scitotenv.2016.10.236.
ABSTRACT:
Glyphosate and atrazine are the most intensively used herbicides in the United States. Although there is abundant spatial and temporal information on atrazine occurrence at regional scales, there are far fewer data for glyphosate, and studies that compare the two herbicides are rare. We investigated temporal patterns in glyphosate and atrazine concentrations measured weekly during the 2013 growing season in 100 small streams in the Midwestern United States. Glyphosate was detected in 44% of samples (method reporting level 0.2μg/L); atrazine was detected above a threshold of 0.2μg/L in 54% of samples. Glyphosate was detected more frequently in 12 urban streams than in 88 agricultural streams, and at concentrations similar to those in streams with high agricultural land use (>40% row crop) in the watershed. In contrast, atrazine was detected more frequently and at higher concentrations in agricultural streams than in urban streams. The maximum concentration of glyphosate measured at most urban sites exceeded the maximum atrazine concentration, whereas at agricultural sites the reverse was true. Measurement at a 2-day interval at 8 sites in northern Missouri revealed that transport of both herbicide compounds appeared to be controlled by spring flush, that peak concentration duration was brief, but that peaks in atrazine concentrations were of longer duration than those of glyphosate. The 2-day sampling also indicated that weekly sampling is unlikely to capture peak concentrations of glyphosate and atrazine.